背景介绍 COT is an oncogene that can activate both the MAP kinase and JNK kinase pathways. COT activates IkappaB kinases and induces the nuclear production of NF-κB. C-terminal catalytic domain of KSR2 associates with COT and KSR2 can negatively regulates the kinase activity of COT in vitro. Co-transfection of KSR2 with COT in cells lead to reduced COT-mediated ERK activation and COT-induced IL8 production in a dose-dependent manner. Cot is one of the MAP kinase kinase kinases that regulates the ERK1/ERK2 pathway in response to IL-1. Blockage of expression of Cot results in failure of IL-1 to induce an increase in IL-8 and MIP-1betamRNA levels. 产品介绍 Human Cot/Tpl2 (GenBank Accession No. NM_005204), (a.a. 30-397), with N-terminal GST-His-tag, MW= 72 kDa, expressed in a Baculovirus infected Sf9 cell expression system.