背景介绍 COT is an oncogene that can activate both the MAP kinase and JNK kinase pathways. COT activates IkappaB kinases and induces the nuclear production of NF-κB. C-terminal catalytic domain of KSR2 associates with COT and KSR2 can negatively regulates the kinase activity of COT in vitro. Co-transfection of KSR2 with COT in cells lead to reduced COT-mediated ERK activation and COT-induced IL8 production in a dose-dependent manner. Cot is one of the MAP kinase kinase kinases that regulates the ERK1/ERK2 pathway in response to IL-1. Blockage of expression of Cot results in failure of IL-1 to induce an increase in IL-8 and MIP-1betamRNA levels. 产品介绍 Mouse Cot kinase (MAP3K8), GenBank Accession No. NM_007746, a.a. 30-397, with N-terminal His-GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. Theoretical MW is 70 kDa.