背景介绍 LSD1 is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3. LSD1 is a critical component of transcriptional regulation via epigenetic histone modifications and is therefore a potential target for drug development. 产品介绍 The LSD1 Homogeneous Assay Kit is designed to measure activity of lysine-specific demethylase (LSD1) for screening and profiling applications. The key to the LSD1 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase detection. First, a sample containing LSD1 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.