背景介绍 JMJD2A is a JmjC-domain protein that exhibits demethylation activity toward H3-K9Me3 and H3-K36Me3. 产品介绍 The JMJD2A Homogeneous Assay Kit is designed to measure activity of the JMJD2A for screening and profiling applications, using AlphaLISA® technology. The key to the JMJD2A Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2A enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.