背景介绍 CARM1 (PRMT4) methylates (mono- and asymmetric dimethylation) the arginine residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. It is recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at Arg17 (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), resulting in transcription activation via chromatin remodeling. CARM also methylates arginines in several other proteins, including EP300/P300 at Arg-2142, Arg-580, and Arg-604. CARM1 acts as coactivator for PPARG, and seems to be involved in p53/TP53 transcriptional activation. 产品介绍 Human PRMT4 (CARM1), GenBank Accession No. NM_199141, a.a. 2-end, with N-terminal FLAG-tag, MW=66 kDa, expressed in FreeStyle 293-F cells.