背景介绍 CD137, also known as 4-1BB, is a prototype co-stimulatory molecule expressed on T cells. CD137 interaction with its cognate ligand, CD137L, also known as 4- 1BBL, results in increased s T-cell activation and proliferation. Accumulating evidence shows a role for CD137:CD137L signaling in inflammation, suggesting that inhibition of this pathway may provide a therapeutic avenue to treat autoimmune and inflammatory diseases. 产品介绍 The CD137:CD137L Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD137:CD137L interaction. The key to this kit is the high sensitivity of detection of biotin-labeled CD137 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD137L is coated on a 96-well plate. Next, CD137-biotin is incubated with CD137L on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.