背景介绍 IDO1/2 and TDO overexpression in tumor cells promotes tryptophan depletion in the microenvironment, resulting in suppression of the T-cell mediated immune response. IDO1/2 and TDO catalyze the breakdown of tryptophan into kynurenine, and subsequently KYNU cleaves kynurenine into anthranilic acid, a precursor of tryptophan. High levels of KYNU have been found in lymphoma, breast, liver, pancreas, and urothelial cancers, and is thought to promote tumor aggressiveness and metastasis. KYNU levels are also elevated in many chronic inflammatory skin diseases such as psoriasis and atopic dermatitis inflammatory, making KYNU attractive as a potential therapeutic and prognostic target. 产品介绍 The Kynureninase (KYNU) Inhibitor Screening Assay Kit is designed to measure human KYNU enzyme inhibition. The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 100 reactions. The KYNU Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are mixed and the reaction is initiated through addition of the substrate 3-hydroxy-DL-kynurenine (3-HK). After a room temperature incubation, activity is determined by measuring the fluorescence of the reaction product (ex. 315 nm, em. 415 nm).