背景介绍 ALKBH5 (AlkB Homolog 5, RNA Demethylase) is an enzyme that removes N6-Methyladenosine (m^6A) from target mRNAs. ALKBH5 has been connected to various cancers, viral replication, and metabolic diseases while also being shown to remove the alkylation damage and restore adenine in DNA. 产品介绍 The ALKBH5 Chemiluminescent Assay Kit is designed to measure ALKBH5 activity for screening and profiling applications. The key to the ALKBH5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated substrate. Signal is inversely related to ALKBH5 demethylase activity. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, ALKBH5 enzyme is incubated with the substrate. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.