背景介绍 ICOS (Inducible T-cell Costimulator, CD278) is a costimulatory molecule of the CD28 cell surface receptor superfamily that is expressed on activated T-cells. ICOS is involved in T-cell responses upon binding with its ligand, ICOSL (also known as B7-H2, CD275), which is normally expressed on B-cells, dendritic cells and monocytes. ICOS expression confers an activated phenotype and a strong suppressive capacity to intra-tumoral regulatory T-cells. In particular, the ICOS/ICOSL pathway is required for optimal antitumor responses mediated by anti-CTLA-4 therapy. 产品介绍 The ICOSL:ICOS[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of ICOS:ICOSL signaling. The key to this kit is the high sensitivity of detection of biotin-labeled ICOS by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, ICOSL is coated on a 96-well plate. Next, ICOS is incubated with ICOSL on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.