背景介绍 The tumor necrosis factor (TNF) receptor family member CD27 (TNFRSF7) and its TNF-like ligand CD70 (CD27L, TNFSF7) are a key costimulatory immune checkpoint signal for lymphocyte activation. Among other effects, this interaction promotes the differentiation of T and B cells and participates in immunoglobulin synthesis. CD27:CD70 interaction has been demonstrated to have important roles in autoimmunity, inflammation, infection and cancer, and antibodies targeting either CD27 or CD70 are in clinical trials for oncology. 产品介绍 The CD27:CD70[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD27:CD70 signaling. This kit comes in a convenient 96- well format, with biotin-labeled CD70, purified CD27, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled CD70 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD27 is coated on a 96-well plate. Next, CD70 is incubated with CD27 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.