背景介绍 CD112 (Poliovirus receptor-related 2, PVRL2), widely expressed on antigen-presenting cells and tumor cells, is the high affinity ligand of CD112R (Poliovirus receptor related immunoglobulin domain containing, PVRIG). CD112-CD112R interaction is a positive immune checkpoint that enhances human T cell response and it has emerged as an attractive therapeutic target for oncology. TIGIT (T cell immunoreceptor with Ig and ITIM domains) and CD226 (also called DNAM-1, DNAX Accessory Molecule-1) are other ligands of CD112. 产品介绍 The CD112R:CD112[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD112R-CD112. The key to this kit is the high sensitivity of detection of biotin-labeled CD112 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD112R is coated on a 96-well plate. Next, CD112 is incubated with CD112R on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.