背景介绍 Tumor necrosis factor receptor 2 (TNFR2, TNFRSF1B or CD120b) is a membrane receptor that binds the pleiotropic pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha). TNFR2 is predominately expressed in cells of the immune system, especially regulatory T cells, and by endothelial cells. TNFR2 exhibits neuroprotective properties and promotes tissue regeneration, making it a promising potential therapeutic target for the treatment of Alzheimer's disease, autoimmune diseases and cancer. 产品介绍 The TNFR2:TNF-alpha[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of TNFR2:TNF-alpha. The key to this kit is the high sensitivity of detection of biotin-labeled TNF-alpha by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, TNFR2 is coated on a 96-well plate. Next, biotinylated TNF-alpha is incubated with TNFR2 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.