背景介绍 V-domain Ig Suppressor of T cell activation (VISTA, or B7-H5) is a type I transmembrane protein that functions as an immune checkpoint. V-Set and Immunoglobulin domain containing 3 (VSIG-3, or IGSF11) is a ligand for VISTA which, when bound on activated T cells, inhibits their proliferation and the production of cytokines and chemokines. VSIG-3 expression is elevated in many cancers, especially glioma and melanoma, so the blockade of the VSIG-3/VISTA pathway represents a promising potential cancer immunotherapy strategy. 产品介绍 The VSIG-3:VISTA[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of VISTA binding to VSIG-3. This kit comes in a convenient 96-well format, with biotin-labeled VISTA, purified VSIG-3, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled VISTA by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, VSIG-3 is coated on a 96-well plate. Next, biotinylated VISTA is incubated with VSIG-3 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.