背景介绍 It is well established that RAS mutations are responsible more than 30% of human cancers and KRAS(G12C) is one of the KRAS mutations that is found frequently in lung and colon cancers. Recent studies discovered a small molecule called AMG510 (Amgen) that can block the KRAS(G12C)-mediated signaling pathway by locking the KRAS conformation in the GDP-bound state. Compounds that affect the nucleotide exchange (GDP to GTP) reaction in KRAS could lead to a novel approach to the inhibition of tumor cell growth in KRAS(G12C)-driven tumors. 产品介绍 The KRAS(G12C) Nucleotide Exchange Assay Kit is designed for screening and profiling of KRAS(G12C) antagonists/inhibitors by using BODIPY-GDP to monitor the GDP or GTP binding status. The kit can be used with two different protocols for greater flexibility, either titrating the inhibitor with fixed GTP concentration or titrating the GTP with fixed inhibitor concentration.