背景介绍 PARP2 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones. 产品介绍 The PARP2 Chemiluminescent Activity Assay kit is designed to measure PARP2 activity for screening and profiling applications. The key to the PARP2 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP2 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP2 biotinylated substrate is incubated with the assay buffer that contains the PARP2 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce a chemiluminescence that can then be measured using a chemiluminescence reader.