背景介绍 PARP14 catalyzes NAD-dependent ADP-ribosylation, and PARP14 overexpression in myeloma cells is associated with disease progression and poor survival rates. 产品介绍 The PARP14 Chemiluminescent Activity Assay Kit is designed to measure PARP14 activity for screening and profiling applications. The key to the PARP14 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP14 reactions. First, histone proteins are coated on a 96-well plate. Next, the biotinylated substrate is incubated with an assay buffer that contains the PARP14 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader