背景介绍 G9a is a histone methyltransferase that exhibits methylation activity toward H3-K9. 产品介绍 The G9a Direct Activity Chemiluminescent Assay Kit is designed to measure G9a activity for screening and profiling applications. The key to the G9a Direct Activity Assay Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.