背景介绍 PRMT1 is a histone methyltransferase that exhibits methylation activity toward H4-R3. 产品介绍 The PRMT1 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT1 activity for screening and profiling applications. The key to the PRMT1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.