背景介绍 5-hydroxymethylcytosine (5-hmC) results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. 5-hmC bases were recently discovered in mammalian DNA, in Purkinje neurons, in granule cells and embryonic stem cells where theyare present at high levels (up to 0.6% of total nucleotides in Purkinje cells). Recent reports indicate that 5-hmC is abundant in brain tissue, especially in areas that are associated with higher cognitive functions. Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests 5-hydroxymethylcytosine may represent a new and unique pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. The most commonly used methodologies (e.g. enzymatic approaches, bisulfite sequencing) do not distinguish 5mc from 5-hmC. The development of specific antibodies appears to be the most powerful way to distinguish and specifically enrich for 5-mC and 5-hmC sequences. 产品介绍 Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH.