背景介绍 SGK3 is a member of the SGK family and encodes a phosphoprotein with a PX (phox homology) domain. PKD1 can phosphorylate and activate SGK3 in vitro (1). A 10-fold increase in PKD1 increases the phosphorylation status of SGK3. When expressed in human embryonic kidney cells, IGF1 and peroxide significantly activate SGK3, and the activation could be reduced by preincubation with inhibitors of PI3 kinase. A yeast 2-hybrid screen found direct interaction between human SGK3 and GSK3β. SGK3 phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels (2). 产品介绍 Recombinant full-length human SGK3, also known as CISK and SGKL, (Genbank Accession No. NM_013257) with an N-terminal GST-tag, expressed in Sf9 insect cells using a baculovirus expression system.