背景介绍 GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the N-terminal end. The sugar moiety is not required for the full spectrum of biological activities. Non-glycosylated and glycosylated GM-CSF show the same activities in vitro. Fully glycosylated GM-CSF is biologically more active in vivo than the non-glycosylated protein. This protein is secreted together with other factors by T-cells and macrophages following cell activation by antigens or mitogens. Approximately 90% of the secreted colony stimulating activities are due to GM-CSF. 产品介绍 Recombinant GM-CSF is a disulfide-linked monomer protein, consisting of 127 amino acids and migrates as an approximately 15 kDa protein under reducing conditions. Optimized DNA sequence encoding Human Granulocyte Macrophage-Colony Stimulating Factor mature chain was expressed in E. coli.