背景介绍 IL-3 is produced mainly by T- cells following cell activation by antigens and mitogens, but also by keratinocytes, NK-cells, mast cells, endothelial cells, and monocytes. The analysis of bacterial- derived recombinant IL-3 shows that glycosylation is not required for the activity of IL-3. IL-3 sequences are evolutionarily not well conserved. Human and murine IL-3 show approximately 29% homology at the protein level while murine and rat IL-3 show approximately 54% homology. IL-3-alpha and IL-3- beta are two isoforms of rat IL-3. IL-3 receptors are expressed on macrophages, mast cells, eosinophils, megakaryocytes, basophils, bone marrow progenitor cells, and various myeloid leukemia cells. IL-3/IL-3 receptor complexes have a Kds of 10^-9 - 10^-10 M. Binding of IL-3 to its receptor causes specific phosphorylation of a 150 kDa membrane glycoprotein. 产品介绍 Recombinant mouse IL-3 is a disulfide-linked monomer consisting of 135 amino acid residues, and migrates as an approximately 15 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding murine Interleukin-3 mature chain was expressed in E. coli.