背景介绍 PDGF is synthesized mainly by megakaryocytes. It is stored in the alpha granules of platelets from which it is released after cell activation of platelets. Platelets synthesize a mixture of the three possible isoforms (BB, AB, AA) while fibroblasts stimulated with EGF synthesize AA homodimers. PDGF receptors are expressed in fibroblasts, osteoblasts, chondroblasts, smooth muscle cells, glial cells, and endothelial cells. Two related receptors, are PDGFRalpha (CD140a) or PDGFRbeta ( CD140b). In contrast to many other cytokines PDGF is not released into the circulation. PDGF binds to several plasma proteins and also to proteins of the extracellular matrix which facilitates local concentration of the factor. The factor functions as a local autocrine and paracrine growth factor. In the adult organism PDGF is involved in wound healing processes. The aberrant expression of PDGF is observed with vascular proliferative diseases such as atherosclerosis. PDGF regulates the synthesis of its own receptor and also influences the expression of membrane receptors for IL-1, EGF, 5-Hydroxytryptamine, LDL, transferrin, and muscarinergic receptor. 产品介绍 Recombinant PDGF-AA is a disulfide-linked homodimeric protein consisting of two 126 amino acid residues, migrates as an approximately 28 kDa protein under non-reducing and as 14 kDa under reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human PDGF-AA mature chain was expressed in E. coli.