背景介绍 Pigment epithelium-derived factor (PEDF), a 50- kDa secreted glycoprotein, is widely expressed throughout the human body, with expression decreasing during human hepatocellular carcinoma and breast cancer progression. PEDF binds to a cell membrane receptor to exhibit multifunctional activity in many cell types but its signaling mechanisms are largely unknown. PEDF exerts anti-angiogenic activity by arresting VEGF- or bFGF-mediated endothelial cell migration, inhibiting capillary morphogenesis, and inducing endothelial cell apoptosis. In human dermal microvascular cells, PEDF induces FasL expression and subsequently activation of caspase-8, which initiates the downstream apoptotic cascade. In human umbilical vein endothelial cells (HUVECs), its induction of apoptosis was shown to depend on p38 MAPK activity and to involve activation of caspases-8 and -9. 产品介绍 Recombinant human pigment epithelium-derived factor (PEDF) is a disulfide-linked monomer protein consisting of 440 amino acid residue subunits, and migrates as an approximately 45k Da protein under non-reducing and reducing conditions in SDS-PAGE. Source: Optimized DNA sequence encoding human PEDF mature chain was expressed in E. coli.