背景介绍 The T-Cell Receptor (TCR) is found on the surface of T-cells and is responsible for recognizing antigens bound to MHC (Major Histocompatibility Complex) molecules. Activation of the TCR results in activation of downstream NFAT signaling. The TCR consists of a heterodimer of two different protein chains, of which the alpha (α) and beta (β) chains are the predominant chains. CRISPR/Cas9 genome editing was utilized to remove the TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) regions of the α and β chains, resulting in loss of TCR expression. 产品介绍 The TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains have both been genetically removed by CRISPR/Cas9 genome editing from recombinant Jurkat cells constitutively expressing the firefly luciferase gene under the control of NFAT response elements.