背景介绍 L-Trp is an essential amino acid necessary for protein synthesis in mammalian cells, and the L-Trp to Kyn pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of LTrp that have immunosuppressive functions. IDO1 is upregulated in many tumors, providing cancer cells with an avenue for immune evasion. 产品介绍 The IDO1 Cellular Activity QuickDetectTM Supplements are designed to complement the hIDO1-HEK293 Recombinant Cell Line (BPS Cat. #60532) or other IDO1- expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of IDO1 and for the analysis and detection of tryptophan 2,3 dioxygenase (IDO1)- catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.