背景介绍 L-Trp is an essential amino acid necessary for protein synthesis in mammalian cells, and the L-Trp to Kyn pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L -Trp that have immunosuppressive functions. IDO2 is upregulated in many tumors, providing cancer cells with an avenue for immune evasion. 产品介绍 The IDO2 Cellular Activity QuickDetect™ Supplements are designed to complement the hIDO2-HEK293 Recombinant Cell Line (BPS Cat. #60532) or other IDO2-expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of IDO2 and for the analysis and detection of indoleamine 2,3 dioxygenase (IDO2)-catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.