背景介绍 L-Trp is an essential amino acid necessary for protein synthesis in mammalian cells, and the L-Trp to Kyn pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. TDO is upregulated in many tumors, providing cancer cells with an avenue for immune evasion. 产品介绍 The TDO Cellular Activity QuickDetectTM Supplements are designed to complement the hTDO-HEK293 Recombinant Cell Line (BPS Cat. #60534) or other TDO-expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of TDO and for the analysis and detection of tryptophan 2,3 dioxygenase (TDO)- catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.